SARS-CoV-2 receptor ACE2 and TMPRSS2 are primarily expressed in bronchial transient secretory cells.
Identifieur interne : 000264 ( Main/Exploration ); précédent : 000263; suivant : 000265SARS-CoV-2 receptor ACE2 and TMPRSS2 are primarily expressed in bronchial transient secretory cells.
Auteurs : Soeren Lukassen [Allemagne] ; Robert Lorenz Chua [Allemagne] ; Timo Trefzer [Allemagne] ; Nicolas C. Kahn [Allemagne] ; Marc A. Schneider [Allemagne] ; Thomas Muley [Allemagne] ; Hauke Winter [Allemagne] ; Michael Meister [Allemagne] ; Carmen Veith [Allemagne] ; Agnes W. Boots [Pays-Bas] ; Bianca P. Hennig [Allemagne] ; Michael Kreuter [Allemagne] ; Christian Conrad [Allemagne] ; Roland Eils [Allemagne]Source :
- The EMBO journal [ 1460-2075 ] ; 2020.
Abstract
The SARS-CoV-2 pandemic affecting the human respiratory system severely challenges public health and urgently demands for increasing our understanding of COVID-19 pathogenesis, especially host factors facilitating virus infection and replication. SARS-CoV-2 was reported to enter cells via binding to ACE2, followed by its priming by TMPRSS2. Here, we investigate ACE2 and TMPRSS2 expression levels and their distribution across cell types in lung tissue (twelve donors, 39,778 cells) and in cells derived from subsegmental bronchial branches (four donors, 17,521 cells) by single nuclei and single cell RNA sequencing, respectively. While TMPRSS2 is strongly expressed in both tissues, in the subsegmental bronchial branches ACE2 is predominantly expressed in a transient secretory cell type. Interestingly, these transiently differentiating cells show an enrichment for pathways related to RHO GTPase function and viral processes suggesting increased vulnerability for SARS-CoV-2 infection. Our data provide a rich resource for future investigations of COVID-19 infection and pathogenesis.
DOI: 10.15252/embj.20105114
PubMed: 32246845
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000355
- to stream PubMed, to step Curation: 000355
- to stream PubMed, to step Checkpoint: 000222
- to stream Ncbi, to step Merge: 003742
- to stream Ncbi, to step Curation: 003742
- to stream Ncbi, to step Checkpoint: 003742
- to stream Main, to step Merge: 000264
- to stream Main, to step Curation: 000264
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">SARS-CoV-2 receptor ACE2 and TMPRSS2 are primarily expressed in bronchial transient secretory cells.</title><author><name sortKey="Lukassen, Soeren" sort="Lukassen, Soeren" uniqKey="Lukassen S" first="Soeren" last="Lukassen">Soeren Lukassen</name><affiliation wicri:level="3"><nlm:affiliation>Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author><author><name sortKey="Chua, Robert Lorenz" sort="Chua, Robert Lorenz" uniqKey="Chua R" first="Robert Lorenz" last="Chua">Robert Lorenz Chua</name><affiliation wicri:level="3"><nlm:affiliation>Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author><author><name sortKey="Trefzer, Timo" sort="Trefzer, Timo" uniqKey="Trefzer T" first="Timo" last="Trefzer">Timo Trefzer</name><affiliation wicri:level="3"><nlm:affiliation>Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author><author><name sortKey="Kahn, Nicolas C" sort="Kahn, Nicolas C" uniqKey="Kahn N" first="Nicolas C" last="Kahn">Nicolas C. Kahn</name><affiliation wicri:level="3"><nlm:affiliation>Department of Pneumology and Respiratory Critical Care Medicine, Center for interstitial and rare lung diseases, Thoraxklinik, Heidelberg University Hospital, Heidelberg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Pneumology and Respiratory Critical Care Medicine, Center for interstitial and rare lung diseases, Thoraxklinik, Heidelberg University Hospital, Heidelberg</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Karlsruhe</region><settlement type="city">Heidelberg</settlement></placeName></affiliation></author><author><name sortKey="Schneider, Marc A" sort="Schneider, Marc A" uniqKey="Schneider M" first="Marc A" last="Schneider">Marc A. Schneider</name><affiliation wicri:level="3"><nlm:affiliation>Translational Lung Research Center Heidelberg (TLRC), Member of the German Center for Lung Research (DZL), Heidelberg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Translational Lung Research Center Heidelberg (TLRC), Member of the German Center for Lung Research (DZL), Heidelberg</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Karlsruhe</region><settlement type="city">Heidelberg</settlement></placeName></affiliation></author><author><name sortKey="Muley, Thomas" sort="Muley, Thomas" uniqKey="Muley T" first="Thomas" last="Muley">Thomas Muley</name><affiliation wicri:level="3"><nlm:affiliation>Translational Lung Research Center Heidelberg (TLRC), Member of the German Center for Lung Research (DZL), Heidelberg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Translational Lung Research Center Heidelberg (TLRC), Member of the German Center for Lung Research (DZL), Heidelberg</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Karlsruhe</region><settlement type="city">Heidelberg</settlement></placeName></affiliation></author><author><name sortKey="Winter, Hauke" sort="Winter, Hauke" uniqKey="Winter H" first="Hauke" last="Winter">Hauke Winter</name><affiliation wicri:level="3"><nlm:affiliation>Translational Lung Research Center Heidelberg (TLRC), Member of the German Center for Lung Research (DZL), Heidelberg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Translational Lung Research Center Heidelberg (TLRC), Member of the German Center for Lung Research (DZL), Heidelberg</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Karlsruhe</region><settlement type="city">Heidelberg</settlement></placeName></affiliation></author><author><name sortKey="Meister, Michael" sort="Meister, Michael" uniqKey="Meister M" first="Michael" last="Meister">Michael Meister</name><affiliation wicri:level="3"><nlm:affiliation>Translational Lung Research Center Heidelberg (TLRC), Member of the German Center for Lung Research (DZL), Heidelberg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Translational Lung Research Center Heidelberg (TLRC), Member of the German Center for Lung Research (DZL), Heidelberg</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Karlsruhe</region><settlement type="city">Heidelberg</settlement></placeName></affiliation></author><author><name sortKey="Veith, Carmen" sort="Veith, Carmen" uniqKey="Veith C" first="Carmen" last="Veith">Carmen Veith</name><affiliation wicri:level="3"><nlm:affiliation>Division of Redox Regulation, German Cancer Research Center (DKFZ) , Heidelberg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Division of Redox Regulation, German Cancer Research Center (DKFZ) , Heidelberg</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Karlsruhe</region><settlement type="city">Heidelberg</settlement></placeName></affiliation></author><author><name sortKey="Boots, Agnes W" sort="Boots, Agnes W" uniqKey="Boots A" first="Agnes W" last="Boots">Agnes W. Boots</name><affiliation wicri:level="1"><nlm:affiliation>Faculty of Health, Medicine and Life Sciences, Department of Pharmacology and Toxicology, NUTRIM School of Nutrition, Translational Research and Metabolism, Maastricht University, Maastricht, the Netherlands.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Faculty of Health, Medicine and Life Sciences, Department of Pharmacology and Toxicology, NUTRIM School of Nutrition, Translational Research and Metabolism, Maastricht University, Maastricht</wicri:regionArea><wicri:noRegion>Maastricht</wicri:noRegion></affiliation></author><author><name sortKey="Hennig, Bianca P" sort="Hennig, Bianca P" uniqKey="Hennig B" first="Bianca P" last="Hennig">Bianca P. Hennig</name><affiliation wicri:level="3"><nlm:affiliation>Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author><author><name sortKey="Kreuter, Michael" sort="Kreuter, Michael" uniqKey="Kreuter M" first="Michael" last="Kreuter">Michael Kreuter</name><affiliation wicri:level="3"><nlm:affiliation>Department of Pneumology and Respiratory Critical Care Medicine, Center for interstitial and rare lung diseases, Thoraxklinik, Heidelberg University Hospital, Heidelberg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Pneumology and Respiratory Critical Care Medicine, Center for interstitial and rare lung diseases, Thoraxklinik, Heidelberg University Hospital, Heidelberg</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Karlsruhe</region><settlement type="city">Heidelberg</settlement></placeName></affiliation></author><author><name sortKey="Conrad, Christian" sort="Conrad, Christian" uniqKey="Conrad C" first="Christian" last="Conrad">Christian Conrad</name><affiliation wicri:level="3"><nlm:affiliation>Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author><author><name sortKey="Eils, Roland" sort="Eils, Roland" uniqKey="Eils R" first="Roland" last="Eils">Roland Eils</name><affiliation wicri:level="3"><nlm:affiliation>Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32246845</idno><idno type="pmid">32246845</idno><idno type="doi">10.15252/embj.20105114</idno><idno type="wicri:Area/PubMed/Corpus">000355</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000355</idno><idno type="wicri:Area/PubMed/Curation">000355</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000355</idno><idno type="wicri:Area/PubMed/Checkpoint">000222</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000222</idno><idno type="wicri:Area/Ncbi/Merge">003742</idno><idno type="wicri:Area/Ncbi/Curation">003742</idno><idno type="wicri:Area/Ncbi/Checkpoint">003742</idno><idno type="wicri:Area/Main/Merge">000264</idno><idno type="wicri:Area/Main/Curation">000264</idno><idno type="wicri:Area/Main/Exploration">000264</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">SARS-CoV-2 receptor ACE2 and TMPRSS2 are primarily expressed in bronchial transient secretory cells.</title><author><name sortKey="Lukassen, Soeren" sort="Lukassen, Soeren" uniqKey="Lukassen S" first="Soeren" last="Lukassen">Soeren Lukassen</name><affiliation wicri:level="3"><nlm:affiliation>Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author><author><name sortKey="Chua, Robert Lorenz" sort="Chua, Robert Lorenz" uniqKey="Chua R" first="Robert Lorenz" last="Chua">Robert Lorenz Chua</name><affiliation wicri:level="3"><nlm:affiliation>Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author><author><name sortKey="Trefzer, Timo" sort="Trefzer, Timo" uniqKey="Trefzer T" first="Timo" last="Trefzer">Timo Trefzer</name><affiliation wicri:level="3"><nlm:affiliation>Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author><author><name sortKey="Kahn, Nicolas C" sort="Kahn, Nicolas C" uniqKey="Kahn N" first="Nicolas C" last="Kahn">Nicolas C. Kahn</name><affiliation wicri:level="3"><nlm:affiliation>Department of Pneumology and Respiratory Critical Care Medicine, Center for interstitial and rare lung diseases, Thoraxklinik, Heidelberg University Hospital, Heidelberg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Pneumology and Respiratory Critical Care Medicine, Center for interstitial and rare lung diseases, Thoraxklinik, Heidelberg University Hospital, Heidelberg</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Karlsruhe</region><settlement type="city">Heidelberg</settlement></placeName></affiliation></author><author><name sortKey="Schneider, Marc A" sort="Schneider, Marc A" uniqKey="Schneider M" first="Marc A" last="Schneider">Marc A. Schneider</name><affiliation wicri:level="3"><nlm:affiliation>Translational Lung Research Center Heidelberg (TLRC), Member of the German Center for Lung Research (DZL), Heidelberg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Translational Lung Research Center Heidelberg (TLRC), Member of the German Center for Lung Research (DZL), Heidelberg</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Karlsruhe</region><settlement type="city">Heidelberg</settlement></placeName></affiliation></author><author><name sortKey="Muley, Thomas" sort="Muley, Thomas" uniqKey="Muley T" first="Thomas" last="Muley">Thomas Muley</name><affiliation wicri:level="3"><nlm:affiliation>Translational Lung Research Center Heidelberg (TLRC), Member of the German Center for Lung Research (DZL), Heidelberg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Translational Lung Research Center Heidelberg (TLRC), Member of the German Center for Lung Research (DZL), Heidelberg</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Karlsruhe</region><settlement type="city">Heidelberg</settlement></placeName></affiliation></author><author><name sortKey="Winter, Hauke" sort="Winter, Hauke" uniqKey="Winter H" first="Hauke" last="Winter">Hauke Winter</name><affiliation wicri:level="3"><nlm:affiliation>Translational Lung Research Center Heidelberg (TLRC), Member of the German Center for Lung Research (DZL), Heidelberg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Translational Lung Research Center Heidelberg (TLRC), Member of the German Center for Lung Research (DZL), Heidelberg</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Karlsruhe</region><settlement type="city">Heidelberg</settlement></placeName></affiliation></author><author><name sortKey="Meister, Michael" sort="Meister, Michael" uniqKey="Meister M" first="Michael" last="Meister">Michael Meister</name><affiliation wicri:level="3"><nlm:affiliation>Translational Lung Research Center Heidelberg (TLRC), Member of the German Center for Lung Research (DZL), Heidelberg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Translational Lung Research Center Heidelberg (TLRC), Member of the German Center for Lung Research (DZL), Heidelberg</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Karlsruhe</region><settlement type="city">Heidelberg</settlement></placeName></affiliation></author><author><name sortKey="Veith, Carmen" sort="Veith, Carmen" uniqKey="Veith C" first="Carmen" last="Veith">Carmen Veith</name><affiliation wicri:level="3"><nlm:affiliation>Division of Redox Regulation, German Cancer Research Center (DKFZ) , Heidelberg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Division of Redox Regulation, German Cancer Research Center (DKFZ) , Heidelberg</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Karlsruhe</region><settlement type="city">Heidelberg</settlement></placeName></affiliation></author><author><name sortKey="Boots, Agnes W" sort="Boots, Agnes W" uniqKey="Boots A" first="Agnes W" last="Boots">Agnes W. Boots</name><affiliation wicri:level="1"><nlm:affiliation>Faculty of Health, Medicine and Life Sciences, Department of Pharmacology and Toxicology, NUTRIM School of Nutrition, Translational Research and Metabolism, Maastricht University, Maastricht, the Netherlands.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Faculty of Health, Medicine and Life Sciences, Department of Pharmacology and Toxicology, NUTRIM School of Nutrition, Translational Research and Metabolism, Maastricht University, Maastricht</wicri:regionArea><wicri:noRegion>Maastricht</wicri:noRegion></affiliation></author><author><name sortKey="Hennig, Bianca P" sort="Hennig, Bianca P" uniqKey="Hennig B" first="Bianca P" last="Hennig">Bianca P. Hennig</name><affiliation wicri:level="3"><nlm:affiliation>Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author><author><name sortKey="Kreuter, Michael" sort="Kreuter, Michael" uniqKey="Kreuter M" first="Michael" last="Kreuter">Michael Kreuter</name><affiliation wicri:level="3"><nlm:affiliation>Department of Pneumology and Respiratory Critical Care Medicine, Center for interstitial and rare lung diseases, Thoraxklinik, Heidelberg University Hospital, Heidelberg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Pneumology and Respiratory Critical Care Medicine, Center for interstitial and rare lung diseases, Thoraxklinik, Heidelberg University Hospital, Heidelberg</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Karlsruhe</region><settlement type="city">Heidelberg</settlement></placeName></affiliation></author><author><name sortKey="Conrad, Christian" sort="Conrad, Christian" uniqKey="Conrad C" first="Christian" last="Conrad">Christian Conrad</name><affiliation wicri:level="3"><nlm:affiliation>Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author><author><name sortKey="Eils, Roland" sort="Eils, Roland" uniqKey="Eils R" first="Roland" last="Eils">Roland Eils</name><affiliation wicri:level="3"><nlm:affiliation>Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author></analytic><series><title level="j">The EMBO journal</title><idno type="eISSN">1460-2075</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The SARS-CoV-2 pandemic affecting the human respiratory system severely challenges public health and urgently demands for increasing our understanding of COVID-19 pathogenesis, especially host factors facilitating virus infection and replication. SARS-CoV-2 was reported to enter cells via binding to ACE2, followed by its priming by TMPRSS2. Here, we investigate ACE2 and TMPRSS2 expression levels and their distribution across cell types in lung tissue (twelve donors, 39,778 cells) and in cells derived from subsegmental bronchial branches (four donors, 17,521 cells) by single nuclei and single cell RNA sequencing, respectively. While TMPRSS2 is strongly expressed in both tissues, in the subsegmental bronchial branches ACE2 is predominantly expressed in a transient secretory cell type. Interestingly, these transiently differentiating cells show an enrichment for pathways related to RHO GTPase function and viral processes suggesting increased vulnerability for SARS-CoV-2 infection. Our data provide a rich resource for future investigations of COVID-19 infection and pathogenesis.</div></front></TEI><affiliations><list><country><li>Allemagne</li><li>Pays-Bas</li></country><region><li>Bade-Wurtemberg</li><li>Berlin</li><li>District de Karlsruhe</li></region><settlement><li>Berlin</li><li>Heidelberg</li></settlement></list><tree><country name="Allemagne"><region name="Berlin"><name sortKey="Lukassen, Soeren" sort="Lukassen, Soeren" uniqKey="Lukassen S" first="Soeren" last="Lukassen">Soeren Lukassen</name></region><name sortKey="Chua, Robert Lorenz" sort="Chua, Robert Lorenz" uniqKey="Chua R" first="Robert Lorenz" last="Chua">Robert Lorenz Chua</name><name sortKey="Conrad, Christian" sort="Conrad, Christian" uniqKey="Conrad C" first="Christian" last="Conrad">Christian Conrad</name><name sortKey="Eils, Roland" sort="Eils, Roland" uniqKey="Eils R" first="Roland" last="Eils">Roland Eils</name><name sortKey="Hennig, Bianca P" sort="Hennig, Bianca P" uniqKey="Hennig B" first="Bianca P" last="Hennig">Bianca P. Hennig</name><name sortKey="Kahn, Nicolas C" sort="Kahn, Nicolas C" uniqKey="Kahn N" first="Nicolas C" last="Kahn">Nicolas C. Kahn</name><name sortKey="Kreuter, Michael" sort="Kreuter, Michael" uniqKey="Kreuter M" first="Michael" last="Kreuter">Michael Kreuter</name><name sortKey="Meister, Michael" sort="Meister, Michael" uniqKey="Meister M" first="Michael" last="Meister">Michael Meister</name><name sortKey="Muley, Thomas" sort="Muley, Thomas" uniqKey="Muley T" first="Thomas" last="Muley">Thomas Muley</name><name sortKey="Schneider, Marc A" sort="Schneider, Marc A" uniqKey="Schneider M" first="Marc A" last="Schneider">Marc A. Schneider</name><name sortKey="Trefzer, Timo" sort="Trefzer, Timo" uniqKey="Trefzer T" first="Timo" last="Trefzer">Timo Trefzer</name><name sortKey="Veith, Carmen" sort="Veith, Carmen" uniqKey="Veith C" first="Carmen" last="Veith">Carmen Veith</name><name sortKey="Winter, Hauke" sort="Winter, Hauke" uniqKey="Winter H" first="Hauke" last="Winter">Hauke Winter</name></country><country name="Pays-Bas"><noRegion><name sortKey="Boots, Agnes W" sort="Boots, Agnes W" uniqKey="Boots A" first="Agnes W" last="Boots">Agnes W. Boots</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000264 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000264 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:32246845
|texte= SARS-CoV-2 receptor ACE2 and TMPRSS2 are primarily expressed in bronchial transient secretory cells.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:32246845" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |